Aijiren Technology, Inc

30/12/2025

6 non-negligible critical points in hplc operation

HPLC Operation Pitfalls to Avoid! 🧪
HPLC users, listen up! Small operational mistakes can ruin your instrument, mess up data, or even cause costly damage. Here are 6 KEY pitfalls to skip & quick fixes to keep your lab work smooth:
🔹 1. Unfiltered Mobile Phase
Pitfall: Dust/impurities scratch core parts & clog pipelines, causing pressure issues.
Fix: Use 0.2μm (normal-phase) / 0.45μm (reverse-phase) syringe filters; add a sand filter at the pump inlet & clean/replace built-in filters regularly.
AIJIREN supply syringe filter in wholesale price, you can contact with whatsapp:+8618336073276.

🔹 2. Skipping Post-Use Pump Cleaning
Pitfall: Salt crystals form from leftover buffer, damaging seals/plungers.
Fix: 2-step clean - flush with ultrapure water first, then switch to storage solvent (methanol for reversed-phase; n-hexane-isopropanol for normal-phase). Never leave corrosive/buffer solutions in the pump long-term.

🔹 3. Mobile Phase Exhaustion
Pitfall: Dry pumping wears parts & causes air bubbles/leaks.
Fix: Check mobile phase level before use; mark min level. If no flow/pressure, purge bubbles (5mL/min flow) or replace worn seals.

🔹 4. Unstable Pressure/Flow
Pitfall: Bubbles or clogged check valves/sand filters.
Fix: Degas mobile phase (15-20min超声/online); purge flow path. Clean check valves with acetone (ultrasonic); unclog sand filters with mobile phase/nitric acid.

🔹 5. Abnormal Pressure (Too High/Low)
Pitfall: High = clogging; Low = leakage.
Fix: Check pipelines, valves, columns & detector flow cell section by section. Flush clogs with suitable solvent; tighten/replace seals at leaks.

🔹 6. Improper Gradient Elution
Pitfall: Solvent stratification, salt crystals, baseline drift.
Fix: Use miscible solvents; test buffer-organic compatibility. Do blank gradient elution first; degas solvents thoroughly.

💡 Pro Tip: HPLC success = detail control! Follow SOPs for mobile phase prep, instrument cleaning & gradient verification to ensure accurate, reproducible data.

25/12/2025

Wishing you a joyful and peaceful holiday season,

Merry Christmas, Happy New Year.
Wishing you a joyful and peaceful holiday season,
In 2026, AIJIREN is on the way to service you and your team.
AIJIREN is your lab chromatography consumable Co-partner.

28/09/2025

AIJIREN is lab chromatography consumable manufacturer

18/08/2025

what is autocalve?

What is Autoclave ? Why is the temperature of autoclaving 121℃？

🔍Let’s clarify two key concepts: Sterilization (the process) and Autoclave (the tool). Sterilization refers to the general process of completely eliminating all microorganisms (including spores) from objects or environments. An Autoclave, on the other hand, is a specialized device used to achieve sterilization—specifically, it uses high-temperature, high-pressure saturated steam for this purpose.

Autoclave sterilization (the sterilization process carried out by an Autoclave) works by leveraging the strong pe*******on and high heat of steam to destroy the core structures of microorganisms (such as proteins and nucleic acids), ultimately achieving "complete sterilization." This method is one of the most commonly used sterilization techniques in biological laboratories, the medical industry, food processing, and other fields.

📌 Microbial Control Falls into 3 Categories—Which One Does Autoclave Sterilization Belong To?​
Methods for controlling microorganisms can be grouped into three main directions, and the role of Autoclave sterilization is clear:​
Killing category: Completely eliminating microorganisms, including "sterilization" (killing all microorganisms, e.g., Autoclave sterilization) and "disinfection" (killing pathogenic microorganisms, e.g., pasteurization);​
Inhibition category: Preventing microbial growth, such as food preservation and pharmaceutical chemotherapy;​
Removal category: Physically separating microorganisms, e.g., filtration.

❓ Key Question: Why Is 121°C Commonly Used for Autoclave Sterilization?​
It is not a "precise calculation requiring exactly 121°C," but a "coincidence" of history and unit conversion related to Autoclave operation:​
Early control via pressure: When Autoclaves were first invented in 1879, there were no temperature sensors, so the Autoclave sterilization process could only be adjusted using pressure gauges. The commonly used pressure standard at that time was 1 kg/cm² (approximately 1 standard atmospheric pressure, i.e., 0.1 MPa), and the boiling point of water at this pressure (a key factor in Autoclave sterilization) is about 119.6°C;​
"Integer preference" in Fahrenheit: Countries like the United States use the Fahrenheit temperature scale, and they tend to set the Autoclave sterilization temperature to 250°F (for its regularity and ease of memory). Converting 250°F to Celsius gives exactly 121.1°C;​
Unified industry standard: Over time, 121°C (paired with 15–20 minutes) has become a globally accepted "default standard" for Autoclave sterilization—it is close to the actual boiling point of water under the target pressure and convenient for Autoclave operation and process recording.

15/08/2025

BCEIA 2025 will be hold in Beijing China.
AIJIREN booth No. E2483, let meet at Beijing.

31/07/2025

Lab sample storage Vial

40mL Sample Storage vial can be also used for TOC and EPA analysis
AIJIREN can supply free sample to test before you order.
whatsapp:+8618336073276

23/07/2025

Lab chromatography consumable supplier

🧪 Are you worried about the quality of lab chromatography consumables in analysis?
Check out these AIJIREN products!​
The sample vials have excellent sealing performance, so you no longer have to worry about sample contamination or leakage, making experimental data more reliable.
The syringe filters and filter membranes have high filtration efficiency, which can quickly obtain pure samples and greatly improve experimental efficiency ⏱️.​
Now, AIJIRNE Sample vial, syringe filter, filter membrane are on hot sale 🔥 with super high cost performance.
They can help you reduce experimental costs while ensuring the accuracy of analysis results.​
Whether it's daily testing or precision experiments, they can meet your needs and make your experiments go more smoothly 🌟!​
E-mail:[email protected] Whatsapp:+8618336073276

26/02/2025

16th Asia Pharam Exhibition is over

23/01/2025

Three major causes of abnormal peak shape in HPLC---01

06/01/2025

Arrival # Sterile Syringe Filters in Various Sizes 0.22 um 0.45 um Pore Sizes 13mm 25mm 33mm Nylon PES PVDF MCE PP CA Membrane filter

Don't miss out! View our new products now!