Hubei Jinjian Biology Co., Ltd

Name: Hubei Jinjian Biology Co., Ltd
Address: No. 38, Qigu Avenue, high tech Changjiang Industrial Park, Xianning, 437300, CN
Telephone: +862763496796

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Hubei Jinjian Biology Co., Ltd

Hubei Jinjian Biology Co., Ltd is a professional biotechnology company who is specialized in development, production and sales of in vitro diagnostic reagents.

22/02/2021

Please kindy find below Novel Coronavirus Neutralizing
Antibody Test Kit catalog for your reference, thanks.

20/02/2021

Congratulations On Hubei Jinjian Biology's Novel Coronavirus Neutralizing Antibody Test Kit Passed the CE Certification.

03/02/2021

Novel Coronavirus Neutralizing Antibody Test Kit (Colloidal Gold Method)

PRINCIPLE：
This kit uses immunochromatography.The test crad contains:1）colloidal gold-labeled recombinant novel coronavirus S-RBD antigen，refer control and quality control antibody gold markers;2）one detection line（T line）,one reference line（R line）and one quality control line（C line）of nitrocellulose membrane.The T line is immobilized with Human ACE2 protein for detecting novel coronavirus neutralizing antibody;the R line is immobilized with reference control antibody;and the C line is immobilized with a quality control antibody.
When an appropriate amount of the test sample is added to the sample hole the test card,the sample will move forward along the test card under the action of the capillary.If the sample contains Novel coronavirus neutralizing antibody,the antibody will bind to the colloidal gold-labeled novel coronavirus antigen.The rest gold-labeled novel coronavirus antigen in immune complex will be captured by the human ACE2 protein immobilized on the membrane to form a purple-red T line,the intensity of T line inversely proportional to the concentration of antibody.
The test card also contains a quality control line C and a reference line R.The fuchsia quality control line C and reference control line R should appear regardless of whether a test line appears.If the quality control line C does not appear,the test result is invalid,and the sample needs to be tested again with another test card.

23/01/2021

Working principle of colloidal gold

Principle:
Use a microporous membrane as a carrier to coat a known antigen or antibody. After adding the sample to be tested, the antibody or antigen in the sample is combined with the antigen or antibody coated on the membrane through the capillary action of the membrane, and then combined with colloidal gold, thus the object achieves the purpose of detection.

Advantage:
1. Fast, Simple
2. Cost effective
3. Stable
4.Suitable for kinds of specimens

21/01/2021

IgM/IgG抗体检测意义：
通常情况下，IgM抗体产生早，一经感染，快速产生，维持时间短，消失快，血液中检测阳性可作为早期感染的指标。IgG抗体产生晚，维持时间长，消失慢，血液中检测阳性可作为感染和既往感染的指标。
IgG抗体检测适合疑似病人筛查，患者治疗效果跟踪，判断痊愈患者是否具有二次感染风险；
IgM比IgG具有更早的升高和下降趋势，并且前期含量高，便于早筛，和治疗效果判断，和IgG型搭配使用。

19/01/2021

"Each category of diagnostic tests has its own unique role in the fight against this virus."

As per the picture, you can choose the suitable test solution:

-7~7 days PCR
-1~7 days antigen
7~14 days IgM, IgG
28 days later IgG

18/01/2021

Why choose the antigen test
Jinjian’s COVID-19 IgG/IgM Rapid Test detects the virus indirectly, but the COVID-19 Antigen Rapid Test detects it directly since it can detect the specific N proteins of the SARS-CoV-2 virus based on the indirect immunochromatography technology. As known, the nucleocapsid protein (N) is ideal for virus detection because it is highly abundant in the virus and sufficiently specific for SARS-CoV-2. It is one of the 4 most important structural proteins of the virus and is involved in the replication, transcription and packaging of the viral genome. Jinjian’s COVID-19 antigen test kit can be used for screening testing in high-risk congregate settings in which repeat testing could quickly identify persons with a SARS-CoV-2 infection to inform infection prevention and control measures, thus preventing transmission.

11/01/2021

Thinking on how B.1.1.7 affect the COVID test kit?-3
Molecular diagnosis: manufacturer's self-examination
Without being affected, the primer and probe design sequences perfectly avoid the S gene mutation position;
And vice versa. I believe there will not be a complete miss, or else I really hit the jackpot.
According to international and national standards, the primers in the kit must identify at least two different genes to minimize false negatives.
But please pay extra attention to the mutation of the new crown, at least the current screening for the new crown in my country is still based on nucleic acid testing. Perhaps the primers of nucleic acid detection kits are not off-target, but this will always require special attention.
A kit that can accurately detect the new crown mutant strain B.1.1.7 is good and everyone needs.

08/01/2021

Thinking on how B.1.1.7 affect the COVID test kit?-2
Antibody testing: basically unaffected
At present, various manufacturers choose S protein and N protein for antibody detection. The same is because S protein is the main surface antigen protein, and N protein is more conservative and uses indirect detection. After the body is infected with the virus, it will produce a variety of antibodies, including different types of antibodies and antibodies that recognize different epitopes.
Again, focus on the impact of sensitivity, the same is the problem of antibody and antigen recognition and binding.

07/01/2021

Thinking on how B.1.1.7 affect the COVID test kit?-1
Antigen test: basically unaffected
The detection antibody is an antibody that recognizes N protein and S protein. The N protein is relatively conservative, and the content is relatively large. According to reports in the literature, a coronavirus particle contains only one RNA, and the average content of its S1 protein and N protein is 222 molecules and more than 3000 molecules.
Pay attention to the impact of sensitivity. Although the mutation on the S protein has not been found to cause major changes in the primary sequence of the antigen for the time being, this means that theoretically the epitope (linear epitope) will not change and the antibody can recognize it normally. However, the primary sequence will lead to secondary structure, and ultimately affect the three-dimensional structure, and finally lead to changes in the binding of antibodies. This is also the most common method of virus escape humoral immunity, and none of them.
The effectiveness of immunodiagnostic reagents lies in the specificity and affinity of the binding of antigen and antibody.

06/01/2021

COVID B.1.1.7

According to the CDC report, the B.1.1.7 virus strain has 23 mutations, including 14 non-synonymous mutations (amino acid changes), 6 synonymous mutations (amino acid does not change) and 3 deletions Mutations; respectively located in ORF1ab gene (4), N gene (2), ORF8 gene (3) and S (Spike) gene (8). Among them, 3 key mutations located on the S gene have potential biological functions.
• N501Y mutation: Located in the S protein receptor binding domain (RBD), the mutation increases the affinity of the virus to the host protein AEC2 receptor.
• 69-70 deletion: It may cause structural changes (ie conformational changes) of the spike protein, which helps the virus escape the host's immune response. Studies have shown that the 69-70 deletion will escape the neutralization effect of some monoclonal antibodies, and may also reduce the neutralization sensitivity of some serums.
• P681H mutation: This site is the 4 key amino acids connecting S1 and S2 in the S protein, which is next to the Furin protease cleavage site. This mutation may cause the S protein to be more easily cleaved by the protease, thereby enhancing its affinity for the AEC2 receptor, Promote the virus to enter the respiratory epithelial cells.

05/01/2021

Please visit our website http://en.jinjianbio.com/
Our linkedin link: https://www.linkedin.com/company/67560267/admin/

Address

No. 38, Qigu Avenue, High Tech Changjiang Industrial Park
Xianning
437300

Telephone

+862763496796

Website

http://en.jinjianbio.com/

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