Chemistry and biology

19/12/2025

Top 10 Cultural Media and Their Key Properties

03/09/2025

Where are the biochemical students?

03/09/2025

Big shout out to my new rising fans! Fatima S Aliyu

21/07/2025

1.Pipette
2. Burette
3. Conical flask
4. Tripod stand
5. Beaker
These tools are for what?

20/07/2025

Shout out to my newest followers! Excited to have you onboard! Rabiyat Muhammad, Ibrahim Sani Sani, Muhammad Ibrahim, Idris Salisu, Zaharaddeen Shuaibu Sarki, Mekyau Ce Ita, Muhammad Yusuf, Mustapha Usman, Salisu Abdurrahaman, Murtala Abubakar, Alhasan A Saadu, Aisha Hussaini, Itz Abdurraxerk Shooaib, Munir Hamza, Hadiza Sunusi, Auwal Usman

02/07/2025

Entamoeba histolytica Antigen Test
1. Objective
The objective of the test was to detect the presence of specific Entamoeba histolytica antigen in stool specimens, assisting in the diagnosis of amoebic dysentery and intestinal amoebiasis.
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2. Principle
The test was based on the detection of E. histolytica-specific antigens using monoclonal antibodies through either an immunochromatographic (rapid card) method or ELISA. If antigens were present in the stool, they reacted with labeled antibodies to form a visible signal or color change.
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3. Materials
• Fresh stool sample
• E. histolytica antigen test kit (ELISA or rapid card)
• Extraction buffer
• Micropipettes or dropper
• Gloves and sterile collection cup
• Incubator (for ELISA method)
• ELISA plate reader (if applicable)
Read more test : https://earthofstory1.blogspot.com/2025/07/antiphospholipid-antibody-test-eg-drvvt.html
4. Procedure (Rapid Immunochromatographic Test)
1. A portion of the stool sample was mixed with the extraction buffer.
2. Several drops of the prepared mixture were added to the sample well of the test cassette.
3. The test cassette was allowed to sit at room temperature for 10–15 minutes.
4. The presence of a test line along with a control line indicated a positive result.
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5. Result (Example)
• Control line only: Negative
• Control + Test line: Positive
• No control line: Invalid
Example:
• Result: Positive
• Interpretation: Entamoeba histolytica antigen detected in stool — confirms amoebic infection.
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6. Uses
• Diagnosed intestinal amoebiasis and amoebic dysentery
• Differentiated between pathogenic E. histolytica and non-pathogenic E. dispar (unlike microscopy)
• Aided in treatment decisions in patients with diarrhea and abdominal pain
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1. Objective: To detect antiphospholipid antibodies in patient plasma, especially in suspected cases of antiphospholipid syndrome, using ...

06/04/2025

Shout out to my newest followers! Excited to have you onboard! Bintu Isah, Hashim Abubakar, Ūsmæn Îßræhîm Mühæmmæm Çîty-ßöy, Abubakar Aliyu, Auwal Abubakar, Aliyu Abdussalam, Abubakar Sadiq, Prince Abdoul, Uwani Mu'azu, Aisha Usman, Abba Na Kano

06/04/2025

✅ Abdominal pain referral areas based on common conditions affecting different organs:

🔷Right Upper Quadrant (RUQ) – Hepatitis, Cholecystitis (Liver and Gallbladder issues).

🔷Epigastric Region (Middle Upper Abdomen) – Peptic ulcer, Pancreatitis (Stomach and Pancreas issues).

🔷Left Upper Quadrant (LUQ) – Splenic injury.

🔷Left Flank (Side Abdomen) – Renal and ureteric pain (Kidney issues).

🔷Periumbilical (Center Abdomen) – Bowel obstruction, Aortic aneurysm (back pain).

🔷Right Flank – Renal and ureteric pain (Kidney issues).

🔷Right Lower Quadrant (RLQ) – Appendicitis.

🔷Suprapubic (Pelvic Region) – Pelvic pain (Ovarian cysts, Pelvic inflammatory disease, etc.).

🔷Left Lower Quadrant (LLQ) – Diverticulitis.

07/11/2022

B.C.H

07/11/2022

Why is it called chemistry?
The word "chemistry" comes from the Arabic word "al-kimia" meaning "the art of transformation". Chemistry began as the study of alchemy. Most alchemists were searching for the "philosopher's stone", a fabled substance that could turn common metals into gold.