Nitric Oxide Services, LLC

10/10/2026

🧪 Nitric Oxide Services, LLC Expands Laboratory Operations to Two Locations!

We are pleased to announce that Nitric Oxide Services, LLC (NOS) now has laboratory operations in Akron, Ohio, and Waxhaw, North Carolina, expanding our ability to support clients throughout the United States and internationally.

With more than 30 years of experience in nitric oxide (NO) research, our mission remains focused on advancing the science of nitric oxide through rigorous analytical characterization, biomedical research, and translational development.

🔬 Our Core Analytical Capabilities Include:✅ Nitric Oxide Donor Characterization – Quantitative assessment of NO-releasing compounds, including release kinetics, concentration-dependent responses, and time-dependent NO release profiles.✅ NO Bioavailability and Metabolite Assessment – Analytical evaluation of nitric oxide, nitrate, nitrite, S-nitrosothiols (RSNOs), and iron–nitrosyl species to better understand NO-related chemistry and biological activity.✅ Biological Matrix Analysis – Assessment of NO-related biomarkers in serum, plasma, urine, cerebrospinal fluid (CSF), and other biological matrices, using matrix-appropriate analytical methods.✅ Method Development and Analytical Validation – Research conducted using rigorous quality-control procedures and analytical validation principles guided by ICH M10, including matrix-matched calibration, assay performance evaluation, and independent quality-control standards.

Why Is This Important?

As the field of nitric oxide therapeutics, drug delivery systems, medical devices, and biomedical applications continues to expand, one fundamental challenge remains: Understanding how much nitric oxide a compound actually releases, under what conditions, and how that NO may become biologically available.

Theoretical NO content alone does not establish actual NO release, bioavailability, or biological activity.Reliable analytical characterization is the essential first step toward understanding these compounds, optimizing their development, and building the scientific foundation necessary for successful clinical translation.

At Nitric Oxide Services, we help bridge the gap between nitric oxide chemistry and biomedical application.We look forward to collaborating with biotechnology companies, pharmaceutical developers, academic investigators, medical device manufacturers, and other innovators advancing the rapidly expanding field of nitric oxide science.

📍 Akron, Ohio | Waxhaw, North Carolina

Nitric Oxide Services, LLCAdvancing Nitric Oxide Science Through Analytical Excellence.

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09/27/2026

Nitric oxide can save a cell—or kill it.

It can promote cancer or help destroy cancer cells.

It can drive inflammation or suppress it.

That paradox is exactly what hooked me when I first encountered nitric oxide in 1994 — and more than 30 years later, it still amazes me.

How can one tiny gaseous free radical produce such opposite biological effects?

The answer often comes down to three things:

📍 Location
📈 Concentration
⏱️ Duration

Change those three variables, and you can change the biology completely.

That is why nitric oxide research can sometimes look contradictory when, in reality, the studies may all be correct.

Same molecule. Different context. Very different outcome.

🧬 In my new blog, I explore what I call the ABCs of Nitric Oxide — three questions that should be asked whenever we try to understand what NO is doing in health or disease.

🔗 https://thebiochemistknowsbest.com/blog-posts/f/🧬-the-abcs-of-nitric-oxide-location-concentration-duration

🧬 The ABCs of Nitric Oxide: Location, Concentration, Duration

09/22/2026

🥬 What if one of the most overlooked benefits of eating vegetables is NITRIC OXIDE? ❤️

We all know fruits and vegetables are good for us. But beyond vitamins, fiber, and antioxidants, there's another fascinating connection: the nitrate–nitrite–nitric oxide (NO) pathway! 🧬

Leafy greens, beetroot, and other vegetables contain naturally occurring nitrate that our bodies can convert into bioactive NO—with a little help from our oral microbiome. 🦠

Why does this matter?

❤️ Supports healthy blood pressure and vascular function

🩸 Helps regulate blood flow and circulation

⏳ May become increasingly important as NO bioavailability declines with age

📚 Clinical studies are revealing how dietary nitrate contributes to cardiovascular physiology, while population studies have linked nitrate-rich vegetable consumption with better cardiovascular outcomes.

Perhaps it's time we looked at that salad a little differently! 🥗

I've explored the science, clinical evidence, and key research in my latest blog:

👇 Beyond Vitamins and Fiber: The Nitric Oxide Connection

https://thebiochemistknowsbest.com/blog-posts/f/%F0%9F%A5%AC-beyond-vitamins-and-fiber-the-nitric-oxide-connection

💡 Food for thought. Science for better health.

🥬 Beyond Vitamins and Fiber: The Nitric Oxide Connection Between Vegetables, Healthy Aging, and Cardiovascular Health

09/18/2026

🧬🧠🔬 What an incredible few days at the University of North Carolina at Charlotte Biomedical Sciences Symposium! 🔬🧠🧬

Just look at this group! 👀📸

It was such a pleasure spending time with so many talented scientists, clinicians, students, entrepreneurs, mentors, and innovators all gathered in one place. The science was exciting, but some of the best moments came from the conversations between sessions — meeting new people, exchanging ideas, finding common research interests, and talking about where collaborations might lead. 🤝💡🧪

One of the highlights for me was also being paired with a new mentee through the symposium mentoring program. 🎓🤝 I’m already excited about the opportunity to stay involved, share experiences from my own career, and — perhaps even more importantly — explore some new science together. There are already some interesting possibilities brewing! 🧫⚗️🚀

A huge THANK YOU 🙏 to the organizing committee at UNC Charlotte, the faculty, students, volunteers, sponsors, the North Carolina Biotechnology Center, and everyone else who helped create such a welcoming and collaborative environment. Events like this remind me why I still love science after all these years. ❤️🔬

And now for the important scientific challenge of the day… 😂

🔎 Where’s Waldo: Biomedical Sciences Edition

Can you find Dr. Joe and Dr. Annette Sysel, American HERO Veterinarian in this picture? 👀😂

Hint: with this many brilliant people in one photograph, it may take a while! 🧠🔍

Looking forward to the new friendships, mentoring relationships, collaborations, and science that come out of this meeting. 🚀

Science is better when we do it together. 🧬🤝🌎

09/18/2026

I’m excited to share that our study, “Fermented Beetroot Supplementation Increases Urinary NOx and Reduces Thromboxane B₂ in eNOS-Deficient Mice,” has now been published in the Journal of Medicinal Food, affiliated with the Korean Society of Food Science and Nutrition.

https://journals.sagepub.com/doi/10.1177/1096620X261489409
This project has been especially meaningful because of the people behind it. It has been a pleasure collaborating with Dae-Sik “Alex” Harm and getting to know Alex and his wife, Yen, who live on Jeju Island, South Korea.

Many people may know Jeju through Lisa See’s The Island of Sea Women, which brought international attention to the remarkable history and culture of Jeju’s haenyeo women divers. Our connection with Jeju has given me an even greater appreciation for the island’s traditions, its food culture, and the opportunities that exist when traditional knowledge is examined through modern biomedical science.

This study evaluated a fermented beetroot preparation in a model with impaired endothelial nitric oxide production. We observed an increase in urinary nitrate/nitrite (NOx) together with a reduction in thromboxane B₂, providing additional evidence that dietary approaches may help support nitric oxide-related pathways even when endogenous eNOS activity is compromised.

What I find particularly exciting is the broader concept: health does not need to be viewed as pharmaceuticals versus nutrition. There is tremendous opportunity to better understand how pharmaceutical interventions, functional foods, dietary strategies, traditional practices, and modern molecular science can complement one another.

This was a pilot study, and there is much more work to do — including defining the active components, understanding the contribution of fermentation, and determining how these findings may translate to human health.

I’m simply proud to have been part of the work and grateful to our collaborators who helped bring it forward.

Joseph A. Bauer, Ph.D.
Annette M. Sysel, DVM, MS
Michael J. Dunphy, Ph.D.
Dae-Sik Harm

Endothelial nitric oxide synthase (eNOS) deficiency reduces nitric oxide (NO) bioavailability and contributes to vascular dysfunction and prothrombotic signalin...

08/27/2026

📢 CALL FOR SUBMISSIONS — JoVE TOPICAL COLLECTION

I am pleased to serve as Guest Editor for a new Journal of Visualized Experiments (JoVE) Topical Collection:

Translational Nitric Oxide Research: Validated Methods for Measurement, Delivery, and Therapeutic Development

We are now seeking submissions in areas including:

🔬 Measurement of nitric oxide and nitrate/nitrite in biological samples

🧪 Chemiluminescence, electrochemistry, spectroscopy, biosensors, and emerging analytical methods

⌚ Validation of wearable and noninvasive NO-related technologies

💊 NO donors, targeted compounds, biomaterials, nanoparticles, topical and inhaled delivery systems

📈 Release kinetics, pharmacokinetics, biodistribution, and pharmacodynamics

🧬 Cancer, cardiovascular and pulmonary disease, wound healing, infectious disease, inflammation, and other translational applications

The central question is:

If an intervention claims to generate, deliver, or alter nitric oxide biology, can we measure it, validate it, determine where it goes, and connect it to a biological or clinical effect?

Why did I take this on?

After more than three decades in experimental and translational research, one problem continues to frustrate me: reproducibility.

How many times have you read the methods section of a paper, tried to reproduce the experiment in your laboratory—and simply could not?

Sometimes the difference between success and failure is a small procedural detail that is difficult to communicate in text alone.

That is what makes JoVE different.

JoVE combines a peer-reviewed scientific manuscript with the opportunity to visually demonstrate how an experiment is actually performed. Their production network can work with researchers to document laboratory procedures, making science more transparent and helping other investigators reproduce and adopt those methods.

I believe visual scientific publishing can significantly improve reproducibility, method transfer, training, collaboration, and ultimately translation.

JoVE accepts Method Articles as well as Research Articles, Case Reports, and Rapid Reports, so submissions are not limited to methods papers.

The standard open access fee is $2,400, which is very reasonable compared with many scientific journals today, particularly given JoVE’s unique visual approach.

Most importantly, I believe this is where scientific publishing is heading.

If you work in nitric oxide biology, analytical chemistry, pharmacology, drug delivery, oncology, vascular biology, biomaterials, wound healing, or translational medicine, I encourage you to consider submitting.

🔗 Collection details and submissions:

https://www.jove.com/methods-collections/5678/translational-nitric-oxide-research-validated-methods-for-measurement-delivery-and-therapeutic-development

Please share with colleagues whose work may be a good fit.

Joseph A. Bauer, PhD, is the founder and executive director of Nitric Oxide Services, LLC, and the founder and treasurer of the Bauer Research Foundation. He has more than three decades of experience in nitric oxide biology, redox chemistry, cancer therapeutics, and translational research. His wo...

08/13/2026

🥊 The Rocky story continues.

I’m pleased to announce that the Nitric Oxide Services, LLC and Bauer Research Foundation, Inc. will be participating in the University of North Carolina at Charlotte Charlotte Regional Biomedical Sciences Symposium 2026: Collaborations Driving Innovations for Impact.

Our work,

“Selective blood-brain barrier pe*******on and tumor targeting of nitrosylcobalamin in glioblastoma: Pharmacokinetics, tissue distribution, and synergistic activity with TRAIL and temozolomide”

has been selected for presentation at this year’s symposium.

For me, this is about much more than another scientific presentation.

The nitrosylcobalamin story has been a long one—years of research, discovery, setbacks, unexpected turns, and plenty of moments when it would have been easier to simply walk away.

But sometimes science requires you to keep getting back up.

That is why I have called this our “Rocky” story.

The science is still standing.

And now, with renewed publications, renewed interest, and new opportunities for collaboration, we believe the next chapter may be just beginning.

The theme of this symposium—Collaborations Driving Innovations for Impact—could not be more appropriate.

The Bauer Research Foundation is actively interested in connecting with investigators, clinicians, institutions, and biotechnology partners working in:

🔬 **Cancer medicine and translational oncology**

🧬 **Nitric oxide biology and therapeutics**

⚗️ **One-carbon metabolism and cobalamin biology**

🧠 **Glioblastoma and blood-brain barrier drug delivery**

🤝 **Translational and combination therapeutic strategies**

No meaningful scientific advance happens in isolation.

If your laboratory, clinical program, institution, or company works in these areas and you see an opportunity to collaborate, I would very much like to hear from you.

Sometimes the next breakthrough begins with a conversation.

See you in Charlotte!

05/25/2026

05/21/2026

For the past 10+ years at Nitric Oxide Services, LLC, nearly every project we’ve worked on has been CUSTOM.

Not “cookie cutter.”
Not “plug-and-play.”
Not a generic CRO workflow recycled from one client to the next.

Custom science.
Custom analytics.
Custom formulation.
Custom FDA-oriented strategy.

That matters when you’re working with one of the most complex and reactive signaling molecules in biology: nitric oxide (NO).

🧪 Our team has worked across an enormous range of nitric oxide technologies and applications, including:

✔ Nitric oxide donors

✔ Nitrite/nitrate delivery systems

✔ Plasma-generated nitric oxide

✔ NO-loaded hydrogels

✔ Water-based NO stabilization systems

✔ Orthopedic and stent coating technologies

✔ Transdermal NO delivery platforms

✔ Exercise physiology & performance applications

✔ Wound healing and regenerative medicine

✔ Nutraceuticals and supplement development

✔ Pharmaceutical reformulation strategies

✔ PK/PD and release kinetics studies

✔ Stability and degradation profiling

✔ FDA-oriented preclinical assay development

We understand:

🔬 HOW nitric oxide is generated

🔬 WHEN it releases

🔬 WHY it degrades

🔬 WHAT conditions stabilize it

🔬 WHICH formulations improve delivery

🔬 HOW regulators will evaluate the data

And we don’t just theorize it.

We have real-world experience with:

⚙ FTIR

⚙ HPLC

⚙ UV-Vis

⚙ Mass spectrometry

⚙ Chemiluminescence nitric oxide analysis

⚙ Temperature and stability chambers

⚙ Controlled release systems

⚙ Custom buffer and pH-release profiling

⚙ Transdermal evaluation models

⚙ Non-animal testing approaches

⚙ GLP/ICH-aligned analytical strategies

One of the biggest mistakes companies make is waiting until AFTER FDA feedback to design the studies they should have done from the beginning.

Our philosophy is different:

➡ Design studies NOW that answer the questions regulators will eventually ask.

That approach has helped many of our clients move forward efficiently while avoiding expensive redevelopment cycles later.

And unlike many large CROs, we work collaboratively and pragmatically.

We build realistic budgets.
We stay flexible.
We communicate directly with clients.

And we tailor the science to YOUR technology.

If your company is developing ANYTHING involving nitric oxide — from pharmaceuticals and medical devices to nutraceuticals, biomaterials, plasma technologies, coatings, or delivery systems — we would love to discuss your project.

📞 Let’s build the right strategy together.

🌐 www.NitricOxideLab.com

04/29/2026

🚨 Featured Publication + Cover Recognition — A Major Milestone 🚨

We’re proud to announce that our latest peer-reviewed publication has been selected as a FEATURED ARTICLE and COVER FEATURE in the IJPC - International Journal of Pharmaceutical Compounding (IJPC) 🎉

👉 You can read the full issue by clicking “View Now” at IJPC.com

📄 Nanoemulsion Reformulation of Nitrosylcobalamin (NO-Cbl) Enhances Solubility, Stability, and Anti-Tumor Potency by Joseph A. Bauer, Ph.D., Dr. Annette Sysel, American HERO Veterinarian, DVM, MS, Bauer Research Foundation, Inc.; Michael Dunphy, Ph.D., Walsh University.

Why this matters 👇

Most people think compounding is simply turning liquids into creams or capsules…

It’s not.

Compounding is one of the most powerful, underappreciated tools in drug development — enabling delivery of therapeutics in ways patients can actually tolerate and use:

✔️ Transdermal instead of injections
✔️ Improved stability for fragile molecules
✔️ Customized dosing and delivery systems

What we demonstrated 🔬

In this study, we tackled a major challenge with nitrosylcobalamin (NO-Cbl) — a promising nitric oxide–based anticancer agent:

➡️ Poor solubility
➡️ Stability limitations
➡️ Delivery constraints

Using a nanoemulsion platform, we showed:

✅ Improved solubility and formulation concentration
✅ Enhanced stability under controlled conditions
✅ ~5-fold increase in anti-tumor potency in melanoma cells

Why this is exciting 💡

This isn’t just about one compound.

This is about a platform approach for difficult molecules — especially those with both hydrophilic and hydrophobic characteristics.

Nanoemulsions may:

✔️ Enable better membrane pe*******on
✔️ Reduce required dose
✔️ Potentially bypass receptor limitations (e.g., CD320 uptake)

✔️ Improve therapeutic outcomes

Our mission

At Nitric Oxide Services, LLC, this is exactly what we do:

🔬 Solve complex formulation challenges
📊 Provide validated analytical support
🧪 Design custom, fit-for-purpose studies
📈 Help move technologies from concept → data → publication → IP

This publication is a perfect example of that pipeline in action.

Let’s build something together

If you’re working on:

• Nitric oxide therapeutics
• Drug delivery challenges
• Topicals, injectables, or nanoformulations
• Wearables or novel platforms

📞 Let’s talk — we’ll help you get to an outcome.