Nitric Oxide Services, LLC

08/27/2026

📢 CALL FOR SUBMISSIONS — JoVE TOPICAL COLLECTION

I am pleased to serve as Guest Editor for a new Journal of Visualized Experiments (JoVE) Topical Collection:

Translational Nitric Oxide Research: Validated Methods for Measurement, Delivery, and Therapeutic Development

We are now seeking submissions in areas including:

🔬 Measurement of nitric oxide and nitrate/nitrite in biological samples

🧪 Chemiluminescence, electrochemistry, spectroscopy, biosensors, and emerging analytical methods

⌚ Validation of wearable and noninvasive NO-related technologies

💊 NO donors, targeted compounds, biomaterials, nanoparticles, topical and inhaled delivery systems

📈 Release kinetics, pharmacokinetics, biodistribution, and pharmacodynamics

🧬 Cancer, cardiovascular and pulmonary disease, wound healing, infectious disease, inflammation, and other translational applications

The central question is:

If an intervention claims to generate, deliver, or alter nitric oxide biology, can we measure it, validate it, determine where it goes, and connect it to a biological or clinical effect?

Why did I take this on?

After more than three decades in experimental and translational research, one problem continues to frustrate me: reproducibility.

How many times have you read the methods section of a paper, tried to reproduce the experiment in your laboratory—and simply could not?

Sometimes the difference between success and failure is a small procedural detail that is difficult to communicate in text alone.

That is what makes JoVE different.

JoVE combines a peer-reviewed scientific manuscript with the opportunity to visually demonstrate how an experiment is actually performed. Their production network can work with researchers to document laboratory procedures, making science more transparent and helping other investigators reproduce and adopt those methods.

I believe visual scientific publishing can significantly improve reproducibility, method transfer, training, collaboration, and ultimately translation.

JoVE accepts Method Articles as well as Research Articles, Case Reports, and Rapid Reports, so submissions are not limited to methods papers.

The standard open access fee is $2,400, which is very reasonable compared with many scientific journals today, particularly given JoVE’s unique visual approach.

Most importantly, I believe this is where scientific publishing is heading.

If you work in nitric oxide biology, analytical chemistry, pharmacology, drug delivery, oncology, vascular biology, biomaterials, wound healing, or translational medicine, I encourage you to consider submitting.

🔗 Collection details and submissions:

https://www.jove.com/methods-collections/5678/translational-nitric-oxide-research-validated-methods-for-measurement-delivery-and-therapeutic-development

Please share with colleagues whose work may be a good fit.

Joseph A. Bauer, PhD, is the founder and executive director of Nitric Oxide Services, LLC, and the founder and treasurer of the Bauer Research Foundation. He has more than three decades of experience in nitric oxide biology, redox chemistry, cancer therapeutics, and translational research. His wo...

08/13/2026

🥊 The Rocky story continues.

I’m pleased to announce that the Nitric Oxide Services, LLC and Bauer Research Foundation, Inc. will be participating in the University of North Carolina at Charlotte Charlotte Regional Biomedical Sciences Symposium 2026: Collaborations Driving Innovations for Impact.

Our work,

“Selective blood-brain barrier pe*******on and tumor targeting of nitrosylcobalamin in glioblastoma: Pharmacokinetics, tissue distribution, and synergistic activity with TRAIL and temozolomide”

has been selected for presentation at this year’s symposium.

For me, this is about much more than another scientific presentation.

The nitrosylcobalamin story has been a long one—years of research, discovery, setbacks, unexpected turns, and plenty of moments when it would have been easier to simply walk away.

But sometimes science requires you to keep getting back up.

That is why I have called this our “Rocky” story.

The science is still standing.

And now, with renewed publications, renewed interest, and new opportunities for collaboration, we believe the next chapter may be just beginning.

The theme of this symposium—Collaborations Driving Innovations for Impact—could not be more appropriate.

The Bauer Research Foundation is actively interested in connecting with investigators, clinicians, institutions, and biotechnology partners working in:

🔬 **Cancer medicine and translational oncology**

🧬 **Nitric oxide biology and therapeutics**

⚗️ **One-carbon metabolism and cobalamin biology**

🧠 **Glioblastoma and blood-brain barrier drug delivery**

🤝 **Translational and combination therapeutic strategies**

No meaningful scientific advance happens in isolation.

If your laboratory, clinical program, institution, or company works in these areas and you see an opportunity to collaborate, I would very much like to hear from you.

Sometimes the next breakthrough begins with a conversation.

See you in Charlotte!

05/25/2026

05/21/2026

For the past 10+ years at Nitric Oxide Services, LLC, nearly every project we’ve worked on has been CUSTOM.

Not “cookie cutter.”
Not “plug-and-play.”
Not a generic CRO workflow recycled from one client to the next.

Custom science.
Custom analytics.
Custom formulation.
Custom FDA-oriented strategy.

That matters when you’re working with one of the most complex and reactive signaling molecules in biology: nitric oxide (NO).

🧪 Our team has worked across an enormous range of nitric oxide technologies and applications, including:

✔ Nitric oxide donors

✔ Nitrite/nitrate delivery systems

✔ Plasma-generated nitric oxide

✔ NO-loaded hydrogels

✔ Water-based NO stabilization systems

✔ Orthopedic and stent coating technologies

✔ Transdermal NO delivery platforms

✔ Exercise physiology & performance applications

✔ Wound healing and regenerative medicine

✔ Nutraceuticals and supplement development

✔ Pharmaceutical reformulation strategies

✔ PK/PD and release kinetics studies

✔ Stability and degradation profiling

✔ FDA-oriented preclinical assay development

We understand:

🔬 HOW nitric oxide is generated

🔬 WHEN it releases

🔬 WHY it degrades

🔬 WHAT conditions stabilize it

🔬 WHICH formulations improve delivery

🔬 HOW regulators will evaluate the data

And we don’t just theorize it.

We have real-world experience with:

⚙ FTIR

⚙ HPLC

⚙ UV-Vis

⚙ Mass spectrometry

⚙ Chemiluminescence nitric oxide analysis

⚙ Temperature and stability chambers

⚙ Controlled release systems

⚙ Custom buffer and pH-release profiling

⚙ Transdermal evaluation models

⚙ Non-animal testing approaches

⚙ GLP/ICH-aligned analytical strategies

One of the biggest mistakes companies make is waiting until AFTER FDA feedback to design the studies they should have done from the beginning.

Our philosophy is different:

➡ Design studies NOW that answer the questions regulators will eventually ask.

That approach has helped many of our clients move forward efficiently while avoiding expensive redevelopment cycles later.

And unlike many large CROs, we work collaboratively and pragmatically.

We build realistic budgets.
We stay flexible.
We communicate directly with clients.

And we tailor the science to YOUR technology.

If your company is developing ANYTHING involving nitric oxide — from pharmaceuticals and medical devices to nutraceuticals, biomaterials, plasma technologies, coatings, or delivery systems — we would love to discuss your project.

📞 Let’s build the right strategy together.

🌐 www.NitricOxideLab.com

04/29/2026

🚨 Featured Publication + Cover Recognition — A Major Milestone 🚨

We’re proud to announce that our latest peer-reviewed publication has been selected as a FEATURED ARTICLE and COVER FEATURE in the IJPC - International Journal of Pharmaceutical Compounding (IJPC) 🎉

👉 You can read the full issue by clicking “View Now” at IJPC.com

📄 Nanoemulsion Reformulation of Nitrosylcobalamin (NO-Cbl) Enhances Solubility, Stability, and Anti-Tumor Potency by Joseph A. Bauer, Ph.D., Dr. Annette Sysel, American HERO Veterinarian, DVM, MS, Bauer Research Foundation, Inc.; Michael Dunphy, Ph.D., Walsh University.

Why this matters 👇

Most people think compounding is simply turning liquids into creams or capsules…

It’s not.

Compounding is one of the most powerful, underappreciated tools in drug development — enabling delivery of therapeutics in ways patients can actually tolerate and use:

✔️ Transdermal instead of injections
✔️ Improved stability for fragile molecules
✔️ Customized dosing and delivery systems

What we demonstrated 🔬

In this study, we tackled a major challenge with nitrosylcobalamin (NO-Cbl) — a promising nitric oxide–based anticancer agent:

➡️ Poor solubility
➡️ Stability limitations
➡️ Delivery constraints

Using a nanoemulsion platform, we showed:

✅ Improved solubility and formulation concentration
✅ Enhanced stability under controlled conditions
✅ ~5-fold increase in anti-tumor potency in melanoma cells

Why this is exciting 💡

This isn’t just about one compound.

This is about a platform approach for difficult molecules — especially those with both hydrophilic and hydrophobic characteristics.

Nanoemulsions may:

✔️ Enable better membrane pe*******on
✔️ Reduce required dose
✔️ Potentially bypass receptor limitations (e.g., CD320 uptake)

✔️ Improve therapeutic outcomes

Our mission

At Nitric Oxide Services, LLC, this is exactly what we do:

🔬 Solve complex formulation challenges
📊 Provide validated analytical support
🧪 Design custom, fit-for-purpose studies
📈 Help move technologies from concept → data → publication → IP

This publication is a perfect example of that pipeline in action.

Let’s build something together

If you’re working on:

• Nitric oxide therapeutics
• Drug delivery challenges
• Topicals, injectables, or nanoformulations
• Wearables or novel platforms

📞 Let’s talk — we’ll help you get to an outcome.

04/18/2026

Not all CROs are built the same.

Most contract research organizations follow a fixed plan.

Once the protocol is signed… you’re locked in.

At Nitric Oxide Services, LLC, we take a different approach:

🔬 We start with your problem — not a template

🧠 We design practical, biologically relevant experiments

🔄 We adapt in real-time as data evolves

🤝 We collaborate with you throughout the project

Because real science isn’t linear—and neither are breakthrough results.

With 30+ years of nitric oxide expertise and PhD-level scientists leading every study, we focus on one thing:

👉 Translating science into practical, real-world applications

From therapeutics and biomaterials to supplements and delivery systems— we help you move from concept → data → impact.

✔ CLIA-certified laboratory

✔ SAM.gov registered

✔ Proven publication & IP track record

If you’re tired of rigid CRO models…

Let’s build something that actually works.

🌐 www.nitricoxidelab.com

04/12/2026

🧪 Unlike many CROs, TEAM NOS does not just run experiments — we help design them.

At Nitric Oxide Services, LLC, discovery and experimental design are part of the value we bring to every client relationship. 🔬 Our GLP and CLIA-certified laboratory specializes in bioanalytical nitric oxide evaluation, and our studies are carried out by trained Ph.D. scientists using proven, tried-and-true SOPs.

📈 We do not hand your project off and disappear.

🤝 We stay in close contact because real science evolves. When results suggest a better path forward, we communicate that promptly rather than rigidly following an outdated plan. That is a major advantage over traditional CRO models.

✅ The TEAM NOS advantage:

🧠 Experimental design and discovery support

👨‍🔬 Ph.D.-level scientific leadership

📋 Proven SOP-driven workflows

🏥 GLP and CLIA-certified capabilities

📞 Ongoing client communication and strategic input

🧬 Deep expertise in nitric oxide science

Behind the scenes, TEAM NOS has supported dozens of Fortune 500 companies while remaining at the forefront of nitric oxide science. 🌎 We also bring a proven track record of peer-reviewed publications that helps guide strategy, inspire innovation, and support client success.

When the science matters, experience matters.

🌐 https://nitricoxidelab.com/

📄 Request for Proposal: https://nitricoxidelab.com/request-for-proposal

04/10/2026

Nitrosylcobalamin (NO-Cbl) may offer a true double hit against cancer.

First, it is designed to exploit CD320-mediated uptake of cobalamin compounds, helping deliver NO-Cbl into tumor cells. Second, our newly published study showed that NO-Cbl can work synergistically with TRAIL and temozolomide, supporting its potential to enhance apoptotic signaling while countering resistance pathways.

In glioblastoma, that matters. Too often, patients receive standard chemotherapy in fixed cycles, only to face tumors that become less responsive over time. By contrast, NO-Cbl was developed as an NO-releasing cobalamin analog that selectively targets tumors through the transcobalamin II receptor (CD320) system, crossed the blood-brain barrier in this study, accumulated in tumor tissue, and showed synergy with both TRAIL and TMZ in glioma cells.

That is why this platform is so important: not just cytotoxic activity, but a strategy that may combine targeted uptake, apoptosis promotion, and suppression of survival signaling in one therapeutic approach. This mechanistic model is part of why we believe NO-Cbl deserves serious attention as a next-generation anticancer strategy.

Proud to share our publication in Oncoscience:

Selective blood-brain barrier pe*******on and tumor targeting of nitrosylcobalamin in glioblastoma: Pharmacokinetics, tissue distribution, and synergistic activity with TRAIL and temozolomide. Published April 2, 2026. DOI: 10.18632/oncoscience.654.

https://www.oncoscience.us/article/654/

04/07/2026

Are you developing a nitric oxide product, technology, or platform?

One of the biggest barriers to success in this space is the lack of rigorous analytical validation. Too many therapeutics, supplements, topicals, wearables, and device-based innovations struggle because the underlying data are not strong enough to support claims, publications, IP, or commercialization.

Nitric Oxide Services, LLC helps solve that problem.

For 10 years, we have supported clients with specialized nitric oxide analytics and translational R&D designed to generate credible, decision-driving data.

Our track record includes contributing to peer-reviewed scientific publications, supporting client IP, and helping innovators build stronger scientific foundations for growth.

If you need a trusted partner in nitric oxide testing and development, let’s talk.

Nitric Oxide Services, LLC
Validated nitric oxide analytics and translational R&D
nitricoxidelab.com