BroadPharm

07/18/2023

Hey LinkedIn Network! We're thrilled to share our latest blog post on "Phosphoramidites: Building Blocks of Oligonucleotide Synthesis"!

In this article, we explore the fascinating world of phosphoramidites, the chemical compounds that play a crucial role in synthesizing oligonucleotides. Discover how phosphoramidites, also known as amidites, enable the covalent bonding of nucleotides, paving the way for the creation of custom oligonucleotide sequences.

Learn about the stepwise synthesis process, automated techniques, and solid-phase synthesis advancements that have revolutionized oligonucleotide production. We also delve into the innovative approach of one-pot-liquid-phase synthesis, presented by the ABMS lab at the University of Freiburg, offering a streamlined and time-saving alternative.

Gain insights into the considerations between traditional stepwise synthesis and the one-pot approach, including factors such as purity, yield, complexity, and specific research or application requirements. Discover which method suits your needs best!

To dive deeper into the world of phosphoramidites and oligonucleotide synthesis, read the full blog post on our website: [insert blog post link]. Don't forget to share your thoughts and leave your valuable comments. We'd love to hear your perspectives!

At BroadPharm, we offer a comprehensive range of phosphoramidites, including DNA, RNA, biotin, fluorescent dye, and Click-Chem phosphoramidites. Our diverse selection empowers researchers worldwide to advance their cutting-edge studies.

A phosphoramidite, also known as an amidite, is a chemical compound used in the synthesis of oligonucleotides, which are short chains of nucleotides. They consist of a nucleoside base (adenine, guanine, cytosine, thymine, or uracil) attached to a phosphoramidite group, which enables covalent bonding...

03/28/2023

Researchers! 🧬 Want to get bright and stable dye-labeled antibodies for your imaging experiments? Check out our Dye Antibody Labeling Kits! Our kits offer a quick and easy way to label antibodies with a range of fluorescent dyes for use in imaging applications.

Our Dye Antibody Labeling Kits enable the simple and efficient labeling of antibodies with a wide range of fluorescent dyes, such as FITC, Alexa Fluor dyes, and Cy dyes. Our kits provide excellent labeling efficiency and retention of antibody activity, making them suitable for various applications, including flow cytometry, microscopy, and immunoassays such as ELISAs.

With our Dye Antibody Labeling Kits, you can:

✅ Visualize your target proteins with bright and stable signals
✅ Use a wide range of fluorescent dyes for different imaging applications
✅ Obtain high-quality imaging data with low background signals

Order now and take the first step towards generating bright and stable dye-labeled antibodies for your imaging experiments!

https://broadpharm.com/product-categories/dye-antibody-labeling-kit

02/13/2023

Maximize the potential of in Drug Delivery! Read our latest blog to discover the science behind this innovative method. A must-read for all in the and vaccines. 💊📚

https://broadpharm.com/blog/lipids-in-drug-delivery

01/31/2023

BroadPharm has recently received a trademark registration number (G958694) for our MagicLink™ kit product line! These biomolecule conjugation kits include oligo, HRP, and alkaline phosphatase antibody conjugation kits, streptavidin conjugation kits, protein-protein crosslinking kits, APC, and PE conjugation kits.

MagicLink™ is BroadPharm’s proprietary technology and PEG crosslinking chemistry. Two target biomolecules are activated by a LINK NHS ester and a MAGIC NHS ester separately and then mixed to yield the MAGIC-LINK backboned conjugates in an hour.

The MagicLink™ conjugation is instant and highly efficient, as seen in the SDS-PAGE below. The linkages are the most stable to date and are resistant to extreme experiment conditions such as high temperature, high/low pH, oxidation, reduction, hydrolysis, etc.

All of our conjugation kits are in stock for overnight shipping
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We also provide timely and high-quality customer conjugation service at an affordable price. You can choose an antibody, enzyme, DNA/RNA, biotin, nanoparticle, quantum dots, fluorescent proteins (e.g., APC, PE), polymer dye (e.g., BV421), tandem dye, fluorescence dye, PEG, etc.- to be conjugated.

Please don’t hesitate to contact [email protected] or call (858) 677-6760 for timely support.

12/16/2022

As 2022 comes to a close, we would like to thank you for all the research that you have done to improve the world. We will continue to support your needs in the new year; warmest thoughts and best wishes this holiday season.

Thank you!
BroadPharm Team
www.broadpharm.com

11/21/2022

Happy Thanksgiving! We have so much to be thankful for this year, most of all we are thankful for your support. Please take a couple minutes to browse our website.

Site --> broadpharm.com

Thank you!
BroadPharm Team

11/17/2022

Nature’s ‘Delete’ button — the cell’s way of sending proteins for recycling — is a protein called Ubiquitin that gets tagged onto proteins by a set of enzymes called E1, E2 and E3 Ubiquitin Ligases. Finding ways to trigger this enzyme cascade is at the heart of cutting-edge research, as sending pathological proteins to the graveyard is one of the safest ways to attack hard-to-reach diseases like cancers or autoimmune diseases. PROTAC — or PROteolysis-TArgeting Chimeras — are small molecules with two functional ends to them, a warhead that binds a target protein of interest and the other that binds an E3 ubiquitin ligase enzyme that marks it for degradation. This one-two combo is excellent for attacking problematic proteins, and research in this area is a hot topic among scientists trying to cure specific cancers or neurodegenerative diseases and so on. At BroadPharm, we help contribute to this research by synthesizing the E3-ligand molecules such as VHL, AHPC, and even full PROTACs like ARV-771. In research settings, PROTACs can be used to selectively degrade a target protein, with no harm to surrounding biomolecules. If one was studying intracellular signaling for example, they could design a ligand molecule targeting Protein Kinase A, and thereby stop all cAMP-related signaling in their cell lines to see what would happen. In clinical settings, where we want to target pathological proteins, a PROTAC ligand can be designed to halt cancer progression by triggering tumor cells for apoptosis by destroying proteins in the Bcl-2 family. In our research highlight this week, the authors designed a PROTAC to do exactly that.
Previously, the authors of “Development of a BCL-xL and BCL-2 dual degrader with improved anti-leukemic activity, | Nature Communications” had successfully created a PROTAC that could target the anti-apoptotic protein, Bcl-xL, in leukemia cells. Their problem though is that in some leukemia cells, both Bcl-2 and Bcl-xL need to be gone in order to kill those cells. By using computers to model the complex formed by Bcl-xL, their PROTAC, a recruiter protein, and the E3 ligase, they realized that the specific lysine that they wanted to target in Bcl-2 just isn’t accessible to the complex. With this information, they redesigned their warhead to better target that specific amino acid. Besides creating amazing figures from their computational modeling, they also did experiments optimizing the fine details for their molecule like linker length, stereochemistry, various mutation experiments to find other key amino acids, with the final product being a new PROTAC that degrades both Bcl-2 and Bcl-xL. With this tool in hand, scientists have another tool in hand to attack leukemia.

Here at BroadPharm we offer a wide range of PROTAC products including aliphatic linkers, BET inhibitors, BTK kinase inhibitors, E3 ligands, and PROTAC linkers. We pride ourselves on product purity and customer satisfaction. Shop our selection today!

Shopping Link→ https://broadpharm.com/product-categories/protac

Lv, D., Pal, P., Liu, X. et al. Development of a BCL-xL and BCL-2 dual degrader with improved anti-leukemic activity,. Nat Commun 12, 6896 (2021). https://doi.org/10.1038/s41467-021-27210-x

11/08/2022

Unconventional drug delivery systems have come to the forefront of the pharmaceutical industry. The groundbreaking COVID-19 vaccine has reignited industry belief that unconventional drug delivery methods are viable. One of these drug delivery methods is akin to ride sharing. By attaching a drug to a protein, they can share a ride to a specific target

Proteins are large, complicated molecules, but the science of linking them is surprisingly simple. All it takes is a nucleophile and an amino acid. The amino acids that like to do this nucleophile chemistry are lysine, with its -NH2 group at the end, and cysteine, with its -SH group at its end. Cysteines have what’s called a ‘thiol’ at the end of them, and these little guys love to get rid of some of their charge by binding to molecules like maleimide. Maleimides have a high affinity for thiols, they react in a unique and highly specific way that binds the protein with maleimide. With this thiol-maleimide reaction, we can link virtually anything we want to our protein or peptide of interest, which is exactly what the authors of our weekly research highlight did.
In the paper, “S2P peptide-conjugated PLGA-Maleimide-PEG nanoparticles containing Imatinib for targeting drug delivery to atherosclerotic plaques,” researchers linked a drug called imatinib to a small peptide. This peptide targets the plaques involved in cardiovascular diseases like stroke. They used a maleimide-PEG linker to do this chemistry, a molecule that is near and dear to us here at BroadPharm. The maleimide end reacted with cysteine residues on both ends of the peptide, binding the peptide to a PEG linker loaded with nanoparticles carrying the imatinib drug on the other end. Through various experimental methods like electron microscopy and H1 NMR, they found that the drug-nanoparticle-peptide conjugate was successfully made and well-characterized. This discovery research can be used down the line to be able to develop new tools to attack plaques in the cardiovascular system, as well as simply inspiring other researchers to use this conjugation method in designing drug targeting systems for the treatment of many different diseases.

BroadPharm cares deeply about how our chemistry can get used in academic research like this, and nothing makes us happier than seeing our work make a difference in the world. Shop our selection of PEG Maleimide Linkers today and empower your research!

https://broadpharm.com/product-categories/peg-linkers/maleimide-linkers

Esfandyari-Manesh, M., Abdi, M., Talasaz, A.H. et al. S2P peptide-conjugated PLGA-Maleimide-PEG nanoparticles containing Imatinib for targeting drug delivery to atherosclerotic plaques. DARU J Pharm Sci 28, 131–138 (2020). https://doi.org/10.1007/s40199-019-00324-w

11/01/2022

Here at BroadPharm we support the research of scientists in pharmaceutical & biotech fields. Today we would like to highlight the accomplishments that have been made in click chemistry.

As scientists working within the molecular world, we often come across challenges in being able to get these tiny units of matter to react how we want them to. In chemistry, purification is often more challenging than making reactions start. That’s why people like Barry Sharpless, Carolyn Bertozzi set out to make a set of reactions that can pop complex groups together in a way that leaves no mess to clean up afterwards. Today, we call this field of study “click chemistry,” and manufacturing materials that make these reactions possible is our specialization here at BroadPharm. One kind of reaction that has gained the spotlight over recent years is the trans cyclooctene / tetrazine (TCO/Tz) click reaction, which is even more specific and fast-paced than the click reactions that came before it. Using this reaction, one can perform chemistry in living systems without disturbing any of a cell’s components, making it perfect for in vivo imaging by ‘clicking’ dyes or radioactive labels onto targets of interest.

Highlighting this reaction is work done by Mengshe Wang and colleagues in their paper “Conformationally Strained trans-Cyclooctene (sTCO) Enables the Rapid Construction of 18F-PET Probes via Tetrazine Ligation.” In this paper, the authors found a way to use TCO/Tz as a part of a radioactive fluorine probe for use in PET imaging, where it can be used to directly see metabolism in living people. By testing their probes in tumors, the authors found it to be a successful method in imaging cancers in humans. This method may lead to earlier detection of cancer and an increase in the therapeutic window.

BroadPharm is a world leading distributor for Click Chemistry Reagents. We provide same day shipping with a focus on customer satisfaction. Check out our click chemistry supply today!!

https://broadpharm.com/product-categories/click-chemistry-reagents

Wang M, Svatunek D, Rohlfing K, Liu Y, Wang H, Giglio B, Yuan H, Wu Z, Li Z, Fox J. Conformationally Strained trans-Cyclooctene (sTCO) Enables the Rapid Construction of (18)F-PET Probes via Tetrazine Ligation. Theranostics. 2016 Apr 12;6(6):887-95. doi: 10.7150/thno.14742. PMID: 27162558; PMCID: PMC4860896.

07/12/2022

BroadPharm has recently launched the MagicLink™ kit product line for biomolecules conjugation. These kits include oligo, HRP and alkaline phosphatase antibody conjugation kits, streptavidin conjugation kit, and protein-protein crosslinking kit, APC, and PE conjugation kits.

MagicLink™ is based on BroadPharm’s proprietary technology and PEG crosslinking chemistry. Two target biomolecules are activated by a LINK NHS ester and a MAGIC NHS ester separately then mixed to yield the MAGIC-LINK backboned conjugates in an hour.

The MagicLink™ conjugation is instant and highly efficient, as seen in the SDS-PAGE below. The linkages are the most stable to date which are resistant to extreme experiment conditions such as high temperature, high/low pH, oxidation, reduction, hydrolysis, etc.

All of our conjugation kits are in-stock for overnight shipping and can be found here: https://broadpharm.com/product-categories/protein-crosslinking-kit

We also provide timely and high-quality customer conjugation service at an affordable price. You can choose antibody, enzyme, DNA/RNA, biotin, nanoparticle, quantum dots, fluorescent proteins (e.g., APC, PE) or polymer dye (e.g. BV421) or their tandem dye, fluorescence dye, PEG, etc. to be conjugated.

Please don’t hesitate to contact [email protected] or call 858-677-6760 for timely support.

06/21/2022

Branched PEG is a class of multi-arm PEG linkers, most notably with three or four arms. PEG linkers are chemically functionalized polyethylene glycol (PEG) compounds which can conjugate with other species such as proteins, antibodies, peptides, RNA, or other chemical entities. Because of their increased size, PEG compounds are filtered by the kidneys slower which increases their half-life in vivo. Hydrated PEG chains also protect compounds from access to proteolytic enzymes and antibodies by steric hindrance and reduce nonspecific degradation.

When utilized in an antibody drug conjugate, branched PEGs also have the ability to attach more than one payload (increasing drug-to-antibody ratio) or targeting moiety to the bioactive molecule of interest. In 2019, research was done by Ma et al. using a BroadPharm Branched PEG to create a dual-targeting molecule that increased the sensitivity of imaging for prostate cancer.

By utilizing a branched PEG linker, Ma’s group attached a prostate-specific antigen targeting moiety, a prostate-specific membrane antigen targeting moiety, and a radiolabeling molecule into one molecule. This dual targeting approach increased sensitivity of prostate cancer diagnostics and laid the groundwork for a new treatment option that would target cancer metastases once the compound was loaded with therapeutic moieties. (source: https://pubs.acs.org/doi/full/10.1021/acs.bioconjchem.9b00252)

BroadPharm offers a wide variety of branched PEG linkers to empower our clients’ research and formulations. Our catalog can be found here: https://broadpharm.com/web/products.php?category1=peg-linkers&category2=branched-peg, and you can also contact us with any questions at [email protected] or (858)677-6760.

06/15/2022

Ionizable lipids are a class of lipid molecules that are neutral at physiological pH and protonated (+) at acidic pH. As one major type of lipid in drug delivery nanoparticles, ionizable lipids facilitate effective RNA delivery by protecting RNA from degradation and assisting in cytosolic transport. RNA therapeutics were not very popular until the success of Onpattro in 2018 (utilizing the ionizable lipid MC3), which re-ignited the enthusiasm for the clinical development of other LNP-based RNA therapeutics, like the mRNA COVID-19 vaccines which utilize SM-102 and ALC-0315 (image below).

In all three lipids, it is apparent that an ester-based biodegradable structure appears in them along with multi/branched-tail and unsaturated structures which facilitate RNA delivery by enhancing membrane disruption, endosomal disruption, and RNA potency.

As a worldwide leading lipid supplier, BroadPharm offers a wide variety of ionizable lipids such as ALC-0315 analogs, SM-102 analogs, MC3, C12-200, and more to empower our clients’ advanced research in nanoparticle drug delivery and RNA therapeutics. Our catalog can be found here: https://broadpharm.com/web/products.php?category1=lipid&category2=ionizable-lipid, please feel free to contact us at +1(858)677-6760 or email [email protected] for any assistance.