GenTarget Inc

GenTarget Inc Lentivirus specialized Biotech company for gene expression, editing, knockDown, knockOut, knockIn.

With more than 20-year biological experiences, including 15-year industry experiences in lentivector development, we have developed many proprietary technologies such as SureTiter Lentiviral system , Optional inducible lentiviral system, Eco instant PCR cloning (Fusion in vivo) method.

Cas Enzymes at a Glance.SpCas9 (Streptococcus pyogenes Cas9):The first and most widely used Cas enzyme. It introduces do...
08/21/2025

Cas Enzymes at a Glance.
SpCas9 (Streptococcus pyogenes Cas9):The first and most widely used Cas enzyme. It introduces double-strand breaks (DSBs) in DNA. Mutant “nickase” versions (D10A, H840A) cut only one strand, making them valuable for reducing off-target effects or for applications like base editing.

SaCas9 (Staphylococcus aureus Cas9): A smaller Cas9, particularly well-suited for AAV delivery due to its compact size.

Cas12a (Cpf1): Unlike Cas9, Cas12a makes staggered (sticky-end) cuts instead of blunt ones. It also requires only a crRNA (no tracrRNA), making it simpler for multiplex editing.

Each Cas enzyme has a specific PAM sequence requirement (e.g., NGG for SpCas9, TTTV for Cas12a) and a distinct gRNA scaffold to ensure accurate DNA targeting. These differences guide researchers in selecting the right Cas for their experiment.

Efficient delivery of Cas enzymes is key to successful genome editing. Lentiviral expression systems provide a reliable and stable method for introducing Cas enzymes—whether SpCas9 (and its dCas9 variants), SaCas9, or Cas12a—into cells.

At Gentarget, we provide ready-to-use Cas expression lentiviruses with advanced features that make CRISPR assays simpler, faster, and more efficient than ever. Read more in link below.

https://www.gentarget.com/product-category/crispr-gene-editing/

01/13/2024

Engineered T cell therapies represent a rapidly growing and dynamic field that has garnered significant attention due to its remarkable effectiveness and breakthroughs in immunotherapy. Two widely employed methods in T cell engineering are Chimeric Antigen Receptor T cell (CAR-T) and TCR-engineered....

CRISPR based epigenomic expression regulationCRISPR/Cas9 gene editing has emerged as a powerful tool for making genomic ...
06/13/2023

CRISPR based epigenomic expression regulation
CRISPR/Cas9 gene editing has emerged as a powerful tool for making genomic sequence changes. However, concerns surrounding off-target effects and undesired alterations in the genome have been raised. To address these challenges, a mutant form of Cas9 known as dCas9 (deficient Cas9 or Dead Cas9) has been developed. Unlike Cas9, dCas9 lacks the ability to cleave double-stranded DNA but can still be guided to specific genomic sites by specific RNA molecules called gRNA.

The fusion of dCas9 with various functional domains allows for precise epigenomic modifications at targeted loci, enabling the modulation of gene expression without altering the underlying DNA sequence. This CRISPR-based gene expression regulation offers several possibilities, including increasing gene expression through linked transcriptional activators (known as CRISPRa), repressing expression through linked methyltransferases for methylation (referred to as CRISPRi), introducing genomic base changes using linked APOBEC enzymes, visualizing specific genomic loci in real-time through fluorescence markers attached to tagged dCas9, and carrying out specific enzymatic reactions at targeted loci using linked enzymes.

One such tool is the “dCas9-Domain” fusion, which has proven to be invaluable for epigenomic analysis. Gentarget, a leading biotechnology company specialized lentivirus products, has developed premade lentivirus products to facilitate various applications in this field. These products have made tasks such as expression repression via KRAB, promoter methylation using different methyltransferases, genomic base changes via APOBEC, genomic base repair through reverse transcriptase, genomic frame shifting using DNA polymerase, expression activation via VP64-SunTag, p65, HSF1, GAL4, and other epigenomic modifications via different domains, easier than ever before.

By leveraging CRISPR-based epigenomic expression regulation, researchers can delve deeper into the complexities of gene regulation and gain a better understanding of how genes are controlled and influenced. We will explore the various applications and advancements in this exciting field, shedding light on the potential of CRISPR technology for epigenomic studies.

Bioluminescent Imaging in Living Cells or Tissue.Fluorescent markers and bioluminescence are two of the most commonly us...
05/03/2023

Bioluminescent Imaging in Living Cells or Tissue.
Fluorescent markers and bioluminescence are two of the most commonly used methods for in vivo imaging in living cells.

Fluorescence imaging is an extremely valuable tool, but it requires external excitation from a laser light. This laser light can have drawbacks such as autofluorescence, phototoxicity, and photobleaching. On the other hand, bioluminescence imaging does not require light activation but suffers from low brightness. It is very dim and typically requires long exposure times, making it impossible to observe small, rapidly moving structures.

To overcome these barriers, an enhanced bioluminescence imaging method called Nano-Lantern was developed by Kenta Saito in 2012. This method uses a bioluminescence resonance energy transfer (BRET) approach to enhance the signal intensity of bioluminescence imaging. Nano-Lantern bioluminescence imaging has an extremely low-level background signal, making it more sensitive and quantitative. When linked to a targeting protein, Nano-Lantern emits light in response to specific biological activity, making it a great tool for visualizing specific biological activity in vivo in real-time.

GenTarget has leveraged its proprietary lentiviral vector systems to create a line of Orange Nano-Lantern Lentivirus products that enable highly sensitive and precise in vivo luminescence imaging. This cutting-edge technology represents a significant improvement over previous methods and is poised to revolutionize many applications with in vivo imaging.

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San Diego, CA

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Monday 9am - 5pm
Tuesday 9am - 5pm
Wednesday 9am - 5pm
Thursday 9am - 5pm
Friday 9am - 5pm

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+18582656446

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