27/10/2026
𝐓𝐂𝐈 𝐏𝐫𝐨𝐝𝐮𝐜𝐭𝐬 𝐇𝐢𝐠𝐡𝐥𝐢𝐠𝐡𝐭𝐬: 𝐏𝐇𝐄𝐌 𝐁𝐮𝐟𝐟𝐞𝐫 𝐟𝐨𝐫 𝐆𝐫𝐞𝐚𝐭 𝐏𝐫𝐞𝐬𝐞𝐫𝐯𝐚𝐭𝐢𝐨𝐧 𝐨𝐟 𝐭𝐡𝐞 𝐂𝐲𝐭𝐨𝐬𝐤𝐞𝐥𝐞𝐭𝐨𝐧 𝐚𝐧𝐝 𝐈𝐧𝐭𝐫𝐚𝐜𝐞𝐥𝐥𝐮𝐥𝐚𝐫 𝐒𝐭𝐫𝐮𝐜𝐭𝐮𝐫𝐞𝐬
5×PHEM Buffer[𝗣𝗿𝗼𝗱𝘂𝗰𝘁 𝗡𝘂𝗺𝗯𝗲𝗿: 𝗣𝟯𝟭𝟳𝟱] is a pH 6.9 buffer consisting of 300 mM PIPES, 125 mM HEPES, 50 mM EGTA, and 10 mM magnesium chloride. It is filter-sterilized and can be used as PHEM buffer after 5-fold dilution with water.
When PHEM buffer was used for cytoskeletal extraction from cultured cells, cytoskeletal structures such as actin filaments and microtubules have been reported to be well preserved, allowing analysis of their three-dimensional organization and structural interactions. Recently, PHEM buffer has also been shown to be useful for sample preparation for electron microscopy.
𝐕𝐢𝐬𝐢𝐭 𝐨𝐮𝐫 𝐰𝐞𝐛𝐬𝐢𝐭𝐞 𝐭𝐨 𝐥𝐞𝐚𝐫𝐧 𝐦𝐨𝐫𝐞: https://www.tcichemicals.com/BE/en/product/tci-topics/ProductHighlights_20261019
𝐅𝐨𝐫 𝐄𝐧𝐪𝐮𝐢𝐫𝐢𝐞𝐬/𝐎𝐫𝐝𝐞𝐫𝐬: 📩 [email protected]