Academy of Contemporary HPLC

Academy of Contemporary HPLC HPLC training courses and books on HPLC troubleshooting and HPLC method development. HPLC Training Course Program Modules

1. Mixed modes
3.

Three-step HPLC method development workflow
2. Adsorption and exclusion HPLC modes. Contemporary HPLC stationary phases
4. Mobile phase composition: reversed-phase HPLC
5. Mobile phase composition: normal-phase HPLC
6. Mobile phase composition: ion-exchange HPLC
7. Mobile phase composition: HILIC & HILIC/ion-exchange HPLC
8. Choosing optimal L, dp and F values
9. Mixed HPLC modes: fine-tuning rete

ntion and selectivity
10. Ion-pair RP HPLC and its alternatives
11. Qualification of HPLC packings
12. Chiral HPLC

Training HPLC Webinars / Seminars / Free Public Lectures

1. No more leakages: how to make the perfect HPLC fitting & capillary choice.
2. Troubleshooting as a misconception: avoiding failures as a better approach to maintenance of HPLC equipment.
3. Excessive column washings as a misconception: a better approach to handling, maintenance and conditioning of silica-based and polymeric HPLC columns.
4. Major types of HPLC method failures: identifying bottlenecks of implemented HPLC methods.

5. Transferability of an HPLC method: how to develop a transferable HPLC method; attributes of non-transferable gradient and isocratic HPLC methods.
6. Quality of an HPLC method: contemporary & outdated HPLC method attributes; consumer attributes of an HPLC method.
7. Quality of an HPLC column: quality of a stationary phase, column packing quality and batch-to-batch reproducibility issues.

8. Application of core-shell HPLC packings for routine applications: arguments pro and con.
9. Ion-pair HPLC and its alternatives: resolving reproducibility and stability issues related to ion-pair HPLC methods; contemporary ion-exchange and mixed reversed-phase / ion-exchange approaches to replace outdated ion-pair HPLC solutions.
10. Matrix-compatible HPLC methods: why sample prep may be unnecessary even for hard-to-handle matrices; better approaches to analyze ointments, suppositories, gels, syrups, pastes, fatty food and oils
11. Specificity & Selectivity: the main characteristics of a routine HPLC method; specific detection conditions and specific HPLC modes; inter- & intra-group selectivity.
12. Chemistries & classification of HPLC stationary phases: problems connected with formal L-classification and outdated selectivity tests developed for HPLC packings; smart HPLC column testing to select truly similar HPLC columns.

13. Performance of a QC lab in focus: identifying the four major technical constraints that hold back its economic growth.

14. Application of HPLC for Drug & Food Quality Control: what is HPLC, how is it used for quality control, numerous examples of drug & food analysis using HPLC.
15. The Future of Analytical HPLC from a Market-Economy Viewpoint: distinguishing features of HPLC as the analytical method from a market-economy viewpoint, economics of HPLC, stagflation of the modern HPLC market and its reasons, advances in HPLC method design, possible HPLC market development scenarios.
16. The design and consumer attributes of an analytical HPLC method: quality of an analytical HPLC method, its quality attributes and consumer attributes, what is method suitability and method validation, drawbacks of the current HPLC method validation procedure, HPLC method cost-effectiveness, complex technical characteristics that affect HPLC method suitability and cost-effectiveness.
17. Advances and problems of contemporary analytical HPLC: distinguishing features of HPLC as the analytical method, problems of contemporary HPLC, the most underestimated HPLC approaches, the most underestimated HPLC detection techniques, advances in development of novel HPLC packings, advances in HPLC method design. Business Consulting

1. Consulting on maximizing throughput and ROI of an HPLC laboratory. Identifying and removing constraints that limit throughput of an analytical HPLC laboratory.

2. Assessment of economic efficiency and suitability of HPLC methods.

3. Consulting on marketing and sales of HPLC columns and HPLC equipment. Development of marketing strategies to promote novel HPLC columns and novel HPLC equipment. Technical Consulting

4. Preparing customized technical purchase specifications for HPLC equipment tailored to the relevant analytical tasks. Selection of the most cost-effective and suitable (U)HPLC methods and state-of-art HPLC columns.

5. Consulting support on any analytical method transfer issues that may arise in connection with API technology transfer.

6. Consulting support on any issues connected with the development of novel HPLC stationary phases, their synthesis and packing technology.

7. Elucidation of HPLC stationary phase chromatographic properties. Finding optimal application fields of HPLC stationary phases and HPLC equipment. (U)HPLC Method Development

1. Our HPLC solutions have a focus on reliability and cost-effectiveness.

2. Routine HPLC methods we provide are specially designed to avoid all possible method failures that can occur during their long-term, extensive use. They are utterly transferable, specific, matrix-compatible, robust and cost-effective.

3. Chromatographic separation and detection conditions are thoroughly fine-tuned to fit the most stringent requirements to resolution, sensitivity and throughput.

4. We put in practice the most recent advances in modern HPLC method design and apply the most up-to-date HPLC stationary phases from well-known manufacturers.

5. In addition to classic RP mode, we widely use various reversed-phase mixed-mode approaches such as RP/ion-exchange, RP/HILIC, RP/CT, as well as classic NP and HILIC modes, various polar mixed modes: HILIC/ion-exchange, HILIC/IEX, NP/CN, etc.

6. We also develop chiral NP, NP/CT, HILIC and RP HPLC methods using the most advanced CSPs present on the market.

Free Library of Separations: Isocratic separation of six water soluble & their phosphates. , , acid, acid, ...
18/08/2024

Free Library of Separations: Isocratic separation of six water soluble & their phosphates. , , acid, acid, -phosphate isomers, -phosphate.

Source: www.hplc.today

📖💡 100 On (from FAQ 42 to FAQ 80):IV. FAQs to an HPLC Method Transfer ManagerIV.42. Why are retention times unstable...
09/11/2023

📖💡 100 On (from FAQ 42 to FAQ 80):
IV. FAQs to an HPLC Method Transfer Manager

IV.42. Why are retention times unstable? How to deal with retention time instability?
IV.43. How to deal with phase collapse (dewetting)?
IV.44. What to do if the peak area is not reproducible?
IV.45. How should you properly inject a sample during manual injection?
IV.46. How to deal with ghost peaks on the chromatogram?
IV.47. What do injectors usually get contaminated with?
IV.48. What should you do if the expected peaks aren't present?
IV.49. What to do if the peak is split? What's the reason for peak splitting?
IV.50. How to fix peak broadening in reversed-phase chromatography?
IV.51. How can a column be overloaded?
IV.52. How does the difference in viscosity between the sample diluent and the mobile phase affect the peak shape?
IV.53. How to address peak broadening and splitting caused by column overload?

V. FAQs to a Technical Support Manager.

V.54. What should be done if the pump system is not working properly?
V.55. How to wash the pump's liquid system?
V.56. How to make sure that the pump works properly?
V.57. How to eliminate the check valve blockage?
V.58. How to replace the pump head? How to replace the inlet check valve?
V.59. What can be done with clogged inlet check valves?
V.60. Why do leaks in the liquid system happen, and how to deal with them?
V.61. High back-pressure: is it dangerous, and how to deal with high back-pressure?
V.62. What to do if the capillary pops out of the column?
V.63. How to deal with high noise when using UV detection?
V.64. How does backpressure regulator work?
V.65. Why does noise increase when the mobile phase absorbs UV light?
V.66. How does the noise depend on wavelength?
V.67. What detector settings affect the noise, and how?
V.68. How to deal with high baseline drift when using UV detection?

VI. Proper Selection of HPLC Stationary Phases

VI.69. Is there a point in introducing a variety of stationary phases to the market?
VI.70. Why are stationary phases with different types of bonding chemistry produced for a single HPLC type?
VI.71. What does "complementary selectivity" mean?
VI.72. Why are more specific modes and stationary phases needed?
VI.73. Why do we need columns of different sizes, meaning different lengths and particle sizes?
VI.74. Are there "best" columns?

VII.

VII.75. What is meant by the "quality" of a column? What makes a column considered high-quality?
VII.76. How are HPLC columns produced?
VII.77. What signs indicate issues in the column packing technology?
VII.78. What does "unstable packing" mean?
VII.79. Is it realistic to get a poorly packed column replaced by a column manufacturer?
VII.80. How can one protect themselves from buying a poorly manufactured column?

eBook: https://lnkd.in/ezefipJu

Paperback version: https://lnkd.in/e6tYurCY

📖💡 100 On (from FAQ 1 to FAQ 40):I.1. How to begin working with a new reversed-phase ?I.2. How does differ from ...
07/11/2023

📖💡 100 On (from FAQ 1 to FAQ 40):
I.1. How to begin working with a new reversed-phase ?
I.2. How does differ from ?
I.3. How to perform the initial washing of a reversed-phase column?
I.4. What volume of mobile phase is sufficient for conditioning a new reversed-phase column?
I.5. How much time is required to condition a new reversed-phase column?
I.6. What should reversed-phase columns be washed with?
I.7. Does it happen that the column is washed for no reason?
I.8. Is there a way to reduce the time spent on washes?
I.9. What precautions need to be taken during column washing?
I.10. What should reversed-phase columns be stored in?
I.11. What causes reversed-phase columns to fail?
I.12. Reasons for the natural wear and tear of reversed phases.
I.13. How can the service life of reversed-phase columns be extended?
I.14. Can reversed-phase columns be regenerated?
I.15. Can you "flip" the column (change its inlet and outlet)?
II.16. What is a buffer solution? How does it work?
II.17. Why do we need different types of buffer solutions?
II.18. What is the purpose of using buffer solutions in HPLC?
II.19. What are the drawbacks of using buffers?
II.20. How to choose the buffer concentration?
II.21. Which buffer is better to use – phosphate or acetate (formate) one?
II.22. Why are mixtures of sodium and potassium salts sometimes used?
II.23. For preparing a phosphate buffer, a mixture of mono- and dibasic phosphates is often used. Why?
II.24. Is it worthwhile to calculate the buffer composition with a specific pH using a formula?
II.25. Why is sodium chloride sometimes added to a phosphate buffer? Can it be avoided?
II.26. What's the purpose of using perchlorates?
II.27. Can a solution of triethylamine or any ion-pairing additive be considered a buffer?
III.28. Why do they sometimes use a mixture of acetonitrile and methanol for preparing the mobile phase?
III.29. Why do they sometimes use mixtures of acetonitrile and THF for preparing the mobile phase?
III.31. Does adding butanol to the mobile phase make sense?
III.32. Why is triethylamine sometimes added to the mobile phase? What are pros and cons?
III.33. How do ion-pair reagents work?
III.34. Which ion-pair reagents are the most popular?
III.35. In which cases ion-pair HPLC is used?
III.36. The main concern with ion-pair separations is that they are poorly transferable. Can anything be done to address this?
III.37. Are ion-pair separations compatible with gradient elution?
III.38. How can ion-pair separations be made more affordable?
III.39. How justified is the use of the ion-pair mode?
III.40. How is separation temperature determined in the reversed-phase mode?

eBook: https://lnkd.in/ezefipJu

Paperback version: https://lnkd.in/e6tYurCY

31/10/2023

HPLC Interview: Questions & Answers, Part 2. The new video is dedicated to the question of volume of the mobile phase (or time) that is necessary to condition a reversed-phase HPLC column. Find more in the book “100FAQs On HPLC”!

eBook: https://www.lulu.com/shop/konstantin-sychev/100-faqs-on-hplc/ebook/product-kvn54r9.html?q=hplc&page=1&pageSize=4

Paperback version: https://www.lulu.com/shop/konstantin-sychev/100-faqs-on-hplc/paperback/product-kvn5ym6.html?q=hplc&page=1&pageSize=4

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Discover the Fast-Track to HPLC Success!📖 Get Your Copy NOW in PDF format! 💻🔥 Dive into 100+ Burning HPLC Questions 🔥No ...
21/10/2023

Discover the Fast-Track to HPLC Success!
📖 Get Your Copy NOW in PDF format! 💻
🔥 Dive into 100+ Burning HPLC Questions 🔥
No More Waiting – Immediate Knowledge Boost!
🔬 Perfect for QC and R&D Pros
👨‍🔬 Beginner-Friendly for Novice Specialists
Don't Miss Out – Elevate Your HPLC Game TODAY! 🌟

Printed version: https://lnkd.in/d4gwfNMS

eBook: https://lnkd.in/dGPKmWfV

What HPLC Webinar Modules do you find the most interesting? Please, specify in the comments.1. Quality of an HPLC Column...
23/06/2022

What HPLC Webinar Modules do you find the most interesting?
Please, specify in the comments.

1. Quality of an HPLC Column: How to Identify a Good / a Poor Quality HPLC Packing / HPLC Stationary Phase
2. How to Select the Alternative to an HPLC Column: Estimating the Retention and the Selectivity for Reversed-Phase, HILIC, and Ion-exchange Stationary Phases
3. Why an HPLC Column Dies: Unveiling the Real Causes of Silica-based & Polymeric Column Failures
4. No More Leakages & HPLC Check Valve Failures: Is It Achievable in Everyday practice of a real-life QC/QA laboratory?
5. Use of Core-shell HPLC Columns for QC Method Development: a Couple of Useful Tips to Ensure the High Performance of Your Routine HPLC Method
6. The Best Practices to Avoid Failures in HPLC Lab: a Comfortable Laboratory Practice vs. HPLC Troubleshooting
7. Complex Matrix Analysis Made Easy: How to Develop a Matrix-Compatible HPLC Method
8. HPLC Method Transferability: Why an HPLC Method Can Be Hard to Transfer, and How to Develop a Transferable HPLC Method
9. Ion-pair HPLC And Its Modern Alternatives: Developing a Robust Ion-pair / Mixed-Mode HPLC Method
10. The Most Underrated HPLC Approaches for Pharmaceutical & Food Analysis
11. How to Develop a Method That Would Be Tolerable to Failures: Improving the ‘Resilience’ of a Routine HPLC Method
12. Top 10 Mistakes in HPLC Method Development. Top 10 Attributes of Outdated HPLC Methods. Top 5 Distinguishing Features a Truly Modern HPLC Method.
13. Why the Cost-effectiveness of an HPLC Method Is Much More Than Just the Analysis Time.
14. Novel Ion-Exchange And Mixed-Mode HPLC Approaches to Pharmaceutical & Food Analysis
15. Innovations in HPLC method design. Consumer attributes and critical quality attributes of an HPLC method. C.E.S.T.R.Us requirements to modern HPLC methods

Good example showing what the true chemical inertness of reversed-phase packing looks like: note peak shapes of & ,...
24/05/2022

Good example showing what the true chemical inertness of reversed-phase packing looks like: note peak shapes of & , they are perfect! Isocratic run, 5um C18 column. Academy of Contemporary HPLC can help you to acquire in-depth knowledge on HPLC method development.

Right now you can subscribe on our upcoming webinars (June 2022) https://www.hplc.today/blog-webinars

Special thanks to Analítica S.A., Manuel Blasco and all Teknokroma team for HPLC columns!

Five that can be met in combinations: the isocratic mixed-mode separation in 6 minutes. Academy of Contemporary HP...
11/05/2022

Five that can be met in combinations: the isocratic mixed-mode separation in 6 minutes.

Academy of Contemporary HPLC can help you to acquire in-depth knowledge on HPLC method development.

Right now you can subscribe on our upcoming webinars and blog posts https://lnkd.in/dgAcFh4X

Thanks to HELIX Chromatography and Vlad Orlovsky for HPLC columns.

That’s how the isocratic separation of three classes of looks on a (SPP) column: about 2 times plate count increas...
10/05/2022

That’s how the isocratic separation of three classes of looks on a (SPP) column: about 2 times plate count increase and also about 2 times throughput increase as compared to the best FPP 5um packing.

Academy of Contemporary HPLC can help you to acquire in-depth knowledge on HPLC method development.

Right now you can subscribe on our upcoming webinars and blog posts https://lnkd.in/dgAcFh4X

Special thanks to Advanced Materials Technology, Harald Richie and all ATM team for HPLC columns!

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