07/11/2017
What is a bioaerosol?
Bioaerosols are airborne particles, solid or liquid. They can be large molecules or volatile compounds. They contain living organisms. They will vary in size from a fraction of a micron to around 100 microns. As with inert “dust” particles, all bioaerosols are governed by the laws of gravity and will be affected by air movements being transported by turbulence and diffusion.
Air will often contain micro-organisms such as viruses, bacteria, and fungi. None of these actually live in the air, the atmosphere tends to kill off most of them. However, they are frequently transported attached to other particles, such as skin flakes, soil, dust, or dried residues from water droplets. Aggregation of cells into clumps can enhance the survival
whilst airborne. Bacterial cells when they become airborne normally rapidly die, within a few seconds, due to evaporation of water associated with the particle. Thus with higher humidity, higher bioaerosol levels can prevail. Airborne fungal cells (yeasts, moulds, spores) can remain viable for much longer periods, even at low relative humidity and high or low temperature extremes. These can pose health risks for humans and animals.
Monitoring of Bioaerosols
Although the use of simple settle plates can be used for collection of bacteria and fungal spores, it can never give a quantitative determination. This passive technique will also fail to enumerate very small particles such as bacteria, which will remain suspended. The simplest quantitative method of monitoring is to use impact samplers such as the MicroBio MB1 or MB2 units. These are single stage impactors, which collect bacteria and fungal spores from air flowing at 100 litres / minute through a series of 1 mm diameter air inlets, onto an agar filled 55 mm contact plate or 90 mm Petri dish, up to a volume of 2,000 litres. The MicroBio samplers are lightweight, battery powered self contained units and do not require an external vacuum pump.
The agar media used should be chosen to suit the organisms which are being monitored. For a wide range of bacteria use tryptic soy agar (TSA), casein soy peptone agar (CPSA) and nutrient agar (NA). There are other selective agars for more specific micro-organisms. For fungi (yeasts and moulds) use is made of malt extract agar (MEA) or rose bengal agar (RBA). After sampling with the MicroBio samplers, the agar plates are incubated for specified times and temperatures (typically 1 to 2 days at 25 to 37 deg C) and the colonies which develop are counted. A correction is applied to the count to allow for the possibility that two organisms going through one sampling hole will result in only one colony growth being observed (positive hole correction). This is determined from tables or using the MicroBio analysis spreadsheets available from this website. From the corrected count and the sampling volume used, the number of colony forming units per cubic metre (CFU/m3) can be determined.
