27/06/2023
𝟳 𝗼𝗳 𝗼𝘂𝗿 𝗺𝗼𝘀𝘁 𝗙𝗔𝗤 𝗶𝗻 𝗿𝗲𝗴𝗮𝗿𝗱 𝘁𝗼 𝗼𝘂𝗿 𝗚𝗲𝗻𝗼𝗺𝗲 𝗟𝗲𝗻𝗴𝘁𝗵 𝗗𝗲𝘁𝗲𝗿𝗺𝗶𝗻𝗮𝘁𝗶𝗼𝗻 𝗠𝗲𝘁𝗵𝗼𝗱.
Vironova BioAnalytics is thrilled to unveil a groundbreaking development in viral vector analysis an innovative, AI-powered method for determining genome length. Pioneered by our dedicated team of scientists, our technique uses cutting-edge cryo-TEM technology, providing a robust and detailed characterization of viral capsids. To the FAQ!
1. What is the scope of the genome length determination method?
The scope of the method is to provide the percentages of different capsid species as well as their genome size estimates. This method also provides the ability to reveal unexpected genome sizes in a sample and to inspect individual particles instead of the bulk sample.
2. What is the required volume and particle concentration of the sample to perform the TEM analysis?
We recommend a minimum sample volume of 25 µL and a buffer volume of 2 mL. However, only 3µL of sample is applied for each grid. The sample concentration is dependent on particle type. For example, AAV samples are recommended to have a concentration > 1012 - 1013 particles per mL for a good on-grid concentration. For other types of particles, we kindly suggest contacting us for discussion.
3. Is the new method considered to be a quantitative method?
Yes, the new method provides the calculated genome size as well as the percentage of each peak or particle population. Our standard analysis is performed using a benchmark of at least 1500 particles per sample.
4. What is the accuracy of the new method?
Currently, the resolution of the method is within ~300 nucleotide bases and we expect that the accuracy will improve with further method development.
5. Is this method suitable for other particle types, including lentivirus, lipid nanoparticles, and bacteriophages?
Yes, we envisage that the method will be suitable for many other particle types. Currently, we can measure each particle’s internal density and give an estimate of its content ratio. However, for more accurate measurements we require reliable reference data and would be very interested to collaborate if you have this data available.
6. If we send you a sample for determining genome length, do you also run a reference standard for each test?
We use qualified equipment as part of our GMP practice. As such, we expect that there will be no significant effects caused by our equipment. However, we can use client-specific references if a client would like to have a reference sample analyzed alongside their test sample.
7. Can the new method be validated for our products?
We can perform client- and product-specific validation studies to assure the reliability, accuracy, and precision of the method.
