09/06/2022
Specific experience:
-bioinformatics database (NCBI, IMGT, SwissPro, ..) searching/blasting
-RNA isolation from mammalian cell RNA isolation and cDNA synthesis
-primer design, PCR, mutagenesis (mutation, insertion, deletion, recombination)
-expression cloning of genes, particularly variable regions of immunoglobulin
-transient expression and stable line generation of 293T and CHO cells
-characterization of recombinantly expressed proteins